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5 (4 g/l) Assay ≥99%
Both FM and SIH media are based on mineral salts in micro and macro concentrations supplemented with several vitamins
Tryptone 35 Yeast Extract 20 NaCl 5 Agar 7 Final pH 7
2 at 25°C Suspend 30 gram powdered medium in 1 litre distilled water
SIH Medium Size:250g 5 (4 g/l) Assay ≥99%SIH medium, developed by Hanh, Friehs and Flaschel (2004), is the next step in the development of synthetic media designed to grow D. discoideum in high cell densities. SIH medium is an improved version of FM medium, as developed by Frank and Kessin (1977). The main difference between SIH and FM is the novel addition of Aspartic acid (1. 1 mM) and increased levels of Lysine (8. 5 mM) and Tryptophane (1. 7 mM). Concentrations of Cysteine, Glutamic